Purification and characterization of lipase enzyme from endophytic Bacillus pumilus WSS5 for application in detergent industry

Lipolytic enzymes from endophytic microbes have been the focus of intense and growing research in view applicability such various industrial applications microorganisms may emerge as a potential source lipases with distinct characteristics. The current work involved purification characterization lipase an endophytic-bacteria isolated stem Withania somnifera L. Dunal. Based on analysis morphology 16S rDNA sequence, bacteria was identified Bacillus pumilus designated B. WSS5. Purified WSS5 found to be 27KDa revealed by SDS-PAGE optimum activity at 37 °C pH 8. stability enzyme presence metal ions, surfactants, inhibitors, organic solvents suggested that this could exploited leather detergent industries. In addition, stain removal crude purified determined cotton fabric pieces stained vegetable oil. Lipase added water along significantly enhanced efficiency commercially available detergent. findings suggest pumilusWSS5 owing promising properties alkali-thermostability, solvent-tolerance, broad substrate specificity makes it strong candidate for application formulations, biotransformation, industries, medicine. To our knowledge, is first report bacterial endophyte